Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The effects of vincristine on WRL68 cells and cell viability were assessed using the CCK-8 method. Cells were treated with different concentrations of vincristine (0.00, 0.01, 0.10, 1.00, 5.00, and 10.00 μg/mL) for 48 h at 37 °C. Data are presented as the means ± SEM determined from five independent experiments. **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: CCK-8 Assay, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The averaged absorption spectra of WRL68 cells that were untreated (control group, black) and treated with 0.120 μg/mL vincristine (treated group, red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The vector-normalized averaged second-derivative spectra of the WRL68 cells that were untreated (control, black) and treated with vincristine (red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the lipid region (3000–2800 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.925, R 2 Y = 0.704, Q 2 = 0.651). The x - and y -axes indicate the first component t1 and the first orthogonal component to1. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean ν as (CH 2 )/ν as (CH 3 ) and ν s (CH 2 )/ν s (CH 3 ) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells, **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from WRL68 cells in the protein region (1800–1480 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.961, R 2 Y = 0.617, Q 2 = 0. 511). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean protein amide I/amide II (1670–1646/1563–1531) and α-helix/β-sheet (1670–1646/1644–1621) absorption band peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells. **** P < 0.0001 and *** P < 0.001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the fingerprint region (1480–900 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.597, R 2 Y = 0.873, Q 2 = 0. 754). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean nucleic acid ν as (PO 2 – )/lipid ester ν(C=O) (1269–1201/1754–1723), nucleic acid ν as (PO 2 – )/protein amide II (1269–1201/1563–1531), nucleic acid ν s (PO 2 – )/lipid ester ν(C=O) (1105–1070/1754–1723), nucleic acid ν s (PO 2 – )/protein amide II (1105–1070/1563–1531), and nucleic acid ν s (dianionic phosphate monoester)/lipid ester ν(C=O) (984–948/1754–1723) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated groups. **** P < 0.0001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: Assignments of the Most Relevant IR Absorption Bands of WRL68 Cells, Together with the Related Vibrational mode 28 − 30

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques:

Journal: Infection and Immunity

Article Title: Mouse Peptidoglycan Recognition Protein PGLYRP-1 Plays a Role in the Host Innate Immune Response against Listeria monocytogenes Infection ▿ †

doi: 10.1128/IAI.00466-10

Figure Lengend Snippet: Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.

Article Snippet: Mouse hepatocyte cell line NMuLi cells (DS Pharma Biomedical, Osaka, Japan) were maintained in DMEM with 10% (vol/vol) FCS.

Techniques: Concentration Assay, Transfection, Control, Plasmid Preparation, Infection, Bacteria, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats

doi: 10.3390/ijms24119230

Figure Lengend Snippet: Bile-derived extracellular vesicles incorporated into the cytoplasm of the hepatic cell line (BRL-3A). Upper left: A phase contrast microscope image. Upper right: A fluorescence microscope image. Mem Dye-Deep Green: Ex 488 nm/Em 490–540 nm. Bottom: An overlay image.

Article Snippet: The cell line derived from normal rat hepatocyte cell line (BRL-3A), obtained from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan), was inoculated to a 6-well plate (1 × 10 5 cells/well), and cultured under 5% CO 2 at 37 °C for 24 h. DMEM, 10% FBS, 1% PS were used as the medium.

Techniques: Derivative Assay, Microscopy, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats

doi: 10.3390/ijms24119230

Figure Lengend Snippet: ( a ) Comparison between the PH-24 group and the sham group for genes reported to be involved in cell cycles. ( A , B ): Genes promoting the G1/S migration ( E2f3 , Dbf4 ). ( C , D ): Genes controlling the centrosome and promoting mitosis in the M phase ( Vsp4a , GEN1 ). ( E ) A gene promoting accurate chromosome segregation in the late M phase ( Chmp4c ). ( F ) A gene constantly acting on and promoting cell cycles ( Ccni ). ( G – I ) Genes whose specific functions are unclear, but are considered to promote cell cycles ( Brsk1 , Dcun1d3 , Mastl ). ( J – M ) Cell cycle checkpoint genes ( Chek2 , Nek6 , Mad2l1 , Ppm1d ). ( N , O ) Genes halting cell cycles (transitioning from G1 phase to G0 phase) ( Anxa1 , Ctdsp2 ). ( P ) A gene demonstrating the proliferative effect at the initial stage of liver regeneration ( Bcl2 ). ( Q ) A gene promoting the induction of the bile acid receptor in the hepatocyte nucleus ( Pias1 ). p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). ( b ) Upregulated genes influencing cell cycles in the PH-24 group. PH: 70% partial hepatectomy.

Article Snippet: The cell line derived from normal rat hepatocyte cell line (BRL-3A), obtained from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan), was inoculated to a 6-well plate (1 × 10 5 cells/well), and cultured under 5% CO 2 at 37 °C for 24 h. DMEM, 10% FBS, 1% PS were used as the medium.

Techniques: Comparison, Migration

Journal: International Journal of Molecular Sciences

Article Title: Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats

doi: 10.3390/ijms24119230

Figure Lengend Snippet: EVs were added to the hepatocyte cell line (BRL-3A), and proliferative ability was verified by the MTS assay. Proliferation was significantly promoted in the PH-24 group than in the sham group and the control group. In addition, in the PH-24 group, proliferation was significantly promoted in the group with EVs added at 1.0 × 10 9 particles/well (dashed line) than in the group with EVs added at 1.0 × 10 8 particles/well (solid line). No difference was observed in the sham group and the control group. PH: 70% partial hepatectomy p < 0.001 (***).

Article Snippet: The cell line derived from normal rat hepatocyte cell line (BRL-3A), obtained from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan), was inoculated to a 6-well plate (1 × 10 5 cells/well), and cultured under 5% CO 2 at 37 °C for 24 h. DMEM, 10% FBS, 1% PS were used as the medium.

Techniques: MTS Assay, Control